Hind-Limb Regeneration in the Dwarf African Clawed Frog, Hymenochirus boettgeri (Anura: Pipidae)

2002 ◽  
Vol 36 (4) ◽  
pp. 537-543 ◽  
Author(s):  
Julie E. Girvan ◽  
Wendy M. Olson ◽  
Brian K. Hall
1983 ◽  
Vol 61 (12) ◽  
pp. 2698-2702 ◽  
Author(s):  
Steven R. Scadding

The objective of this experiment was to determine the effect of retinoic acid on the process of limb regeneration in the African clawed frog, Xenopus laevis. Limbs were bilaterally amputated through the radio-ulna and then treated for 15 days with retinoic acid in the aquarium water, at 3, 15, or 75 IU/mL. The retinoic acid inhibited limb regeneration in a dose-dependent manner, reduced the length of the regenerates, and produced irregularities in the morphogenesis of the cartilage rod in the regenerate. The regenerated limbs were removed after 150 days by amputation through the humerus, and the limbs were again allowed to regenerate. In the retinoic acid treated animals, despite the fact that retinoic acid treatment had been discontinued over 4 months previously, limb regeneration was still inhibited. These results suggest that retinoic acid has a long-term effect on the treated animals.


2015 ◽  
Vol 6 (1/2) ◽  
Author(s):  
Federico Marrone ◽  
Luigi Naselli-Flores

This paper reviews the available knowledge about faunal xenodiversity in Sicilian inland waters (Italy). The aim is to provide an updated checklist and bibliography of those non-indigenous species (NIS) which occur in the island, and to identify possible threats to its native biological diversity. Data were collected through an extensive literature search which encompassed also local journals, books, congress abstracts, and other grey literature. All the collected data were critically revised and, when possible, verified by consulting available collections or through dedicated sampling surveys. Only those data contained in reports indicating precise occurrence localities, which were confirmed by our own observations and\or by at least two independent sources including at least a peer-reviewed publication, were considered as certain. Data in literature that did not meet these criteria were considered doubtful and reported separately as unverified. The information provided by websites has been excluded as it often contains unfounded and\or erroneous data. The fauna of Sicilian inland waters host at present 31 confirmed NIS. In addition, the presence of further 11 taxa is dubious. Among the v<em>erified data</em>, invertebrate and vertebrate taxa are nearly equally represented, with 15 and 16 taxa, respectively. With 16 species, the phylum Chordata is by far the most represented, followed by Mollusca (8 species) and Arthropoda (6 species). Most of these species were detected in the last 30 years due to the lack of previous regular studies on Sicilian freshwaters. With few exceptions (<em>e.g</em>., the recent introduction of <em>Xenopus laevis</em>, the African clawed frog), NIS’ effects on native biota have not extensively studied in the island yet. Although the top-down effects caused by introduced vertebrate taxa are known to deeply modify the native structure of the biota, little information is available on the impacts caused by invertebrate taxa, especially the microscopic ones. The presence in Sicily of 11 nonnative species of bony fish is probably the most impacting threat to autochthonous fauna through predation, competition and hybridisation. The results shown in the paper highlight the importance and the urgency of more exhaustive investigations on NIS in Sicilian freshwaters with special regard to less charismatic taxa whose effects on the native biota have never been evaluated yet.


2000 ◽  
Vol 38 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Christopher A Pearl ◽  
Misty Cervantes ◽  
Monica Chan ◽  
Uyen Ho ◽  
Rane Shoji ◽  
...  

2007 ◽  
Vol 292 (5) ◽  
pp. R1916-R1925 ◽  
Author(s):  
Monika Sundqvist

Little is known about the purinergic regulation of intestinal motor activity in amphibians. Purinergic control of intestinal motility is subject to changes during development in mammals. The aim of this study was to investigate purinergic control of intestinal smooth muscle in the amphibian Xenopus laevis and explore possible changes in this system during the developmental phase of metamorphosis. Effects of purinergic compounds on mean force and contraction frequency in intestinal circular muscle strips from prometamorphic, metamorphic, and juvenile animals were investigated. Before metamorphosis, low concentrations of ATP reduced motor activity, whereas the effects were reversed at higher concentrations. ATP-induced relaxation was not inhibited by the P2-receptor antagonist pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS) but was blocked by the ecto-nucleotidase inhibitor 6- N, N-diethyl-d-β,γ-dibromomethylene ATP ( ARL67256 ), indicating that an ATP-derived metabolite mediated the relaxation response at this stage. Adenosine induced relaxation before, during, and after metamorphosis, which was blocked by the A1-receptor antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX). The stable ATP-analog adenosine 5′-[γ-thio]-triphosphate (ATPγS) and 2-methylthioATP (2-MeSATP) elicited contractions in the circular muscle strips in prometamorphic tadpoles. However, in juvenile froglets, 2-MeSATP caused relaxation, as did ATPγS at low concentrations. The P2Y11/P2X1-receptor antagonist NF157 antagonized the ATPγS-induced relaxation. The P2X-preferring agonist α-β-methyleneadenosine 5′-triphosphate (α-β-MeATP) evoked PPADS-sensitive increases in mean force at all stages investigated. This study demonstrates the existence of an adenosine A1-like receptor mediating relaxation and a P2X-like receptor mediating contraction in the X. laevis gut before, during, and after metamorphosis. Furthermore, the development of a P2Y11-like receptor-mediated relaxation during metamorphosis is shown.


2008 ◽  
Vol 23 (1) ◽  
pp. 131-144 ◽  
Author(s):  
Chun-Sik Yoon ◽  
Jung-Hyo Jin ◽  
Joo-Hung Park ◽  
Chang-Yeol Yeo ◽  
Song-Ja Kim ◽  
...  

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